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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene is noticed in many tumors, which leads to chromosome instability, and thus may well lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a crucial role in promoting cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been described. Two more splice variants ended up documented but thorough nucleotide sequence information and facts is not yet out there.

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Blocking peptides are peptides that bind specially into the focus on antibody and block antibody binding. These peptide commonly contains the epitope identified because of the antibody.

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This tutorial outlines even more probable optimization of cellular and immunoassay parameters to guarantee the absolute best effects are...

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This information outlines further feasible optimization of mobile and immunoassay parameters to make sure the best possible benefits are...

Antibodies bound to the blocking peptide not bind towards the epitope to the concentrate on protein. By evaluating the staining in the blocked antibody vs . the antibody on your own, you can see which staining is precise.

Target Info Cyclin E belongs for the hugely conserved cyclin relatives, whose users are characterised by a extraordinary periodicity in protein abundance with the cell cycle. Cyclins perform as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation styles which add into the temporal coordination of each and every mitotic event. Cyclin E types a posh with and capabilities to be a regulatory read more subunit of CDK2, whose exercise is necessary for cell cycle G1/S transition. This protein accumulates within the G1-S section boundary and is particularly degraded as cells progress by means of S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...

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